Array technology

What is printed on the EmbryoCellect™ microarray?

The EmbryoCellect™ microarray is fundamentally different to other microarrays.

Unlike BAC or oligonucleotide arrays, where each probe contains a single DNA target ranging in size from approximately 60-150 basepairs, each feature (spot) on the EmbryoCellect™ array contains a whole chromosome library. This provides on average 1.2 million unique chromosome-specific target fragments of DNA ranging in size from approximately 200 to 4,000 basepairs.

This approach (single cell microarray to detect aneuploidy in embryos) was first described by RHS scientists from the Department of Obstetrics and Gynaecology, The University of Adelaide in 2004 1 and 2007 2 and is exclusively licensed to RHS.

This patented approach allows the EmbryoCellect™ microarray to collect test and reference signal from an entire chromosome in a single result providing a clear indication of whole chromosome count.

  1. Aneuploidy detection in single cells using DNA array-based comparative genomic hybridization. Hu, D. G., Webb, G., & Hussey, N. Molecular Human Reproduction 2004, 10(4), 283–9.
  2. Gender determination and detection of aneuploidy in single cells using DNA array-based comparative genomic hybridization. Hu, D. G., Guan, X. Y., & Hussey, N. Methods in Molecular Medicine 2007, 132, 135–151.

Multiple copies of single metaphase chromosomes are laser captured.

Individual chromosomes are whole genome amplified using the RHS DOP-PCR and then repeat depleted and printed.

Whole chromosome probes containing on average 1.2 million unique sequences per chromosome are printed onto microarray slides. There are 4 microarrays (test areas) per slide, each microarray containing probes for all 24 human chromosomes in replicates of 8.

Image of an EmbryoCellect™ microarray

Product attributes

  • What is printed on the array?

    Chromosome-specific DOP-amplified and repeat deleted PCR products ranging in size from 120bp – 4kb

  • How are they printed?

    Spot printed as a pooled library of sequences specific to each chromosome

  • Number of array targets

    Over 35 million sequences per array with, on average, 1.2 million chromosome specific sequences per spot.

  • Replicate targets on chip

    One spot for each of the 24 human chromosomes; Eight replicates per array

  • Microarrays per slide

    Four allowing the testing of as few as four samples at a time. One operator can manage 4 slides in a batch if required

  • Ease of analysis

    There is a single spot per chromosome so there is no need to calculate a consensus across the chromosome to detect aneuploidy. The results are very clear and specific for the detection of whole chromosome aneuploidy.