EmbryoCellect®

EmbryoCellect® uses microarray technology (array Comparative Genomic Hybridisation or aCGH) to compare the number of chromosomes in a sample cell to a known reference sample. The samples are labelled with different fluorescent dyes and the amount of fluorescence is measured and compared for each chromosome. 

This provides a reliable and straightforward way to count the number of chromosomes in the test cell and detect whole chromosome aneuploidy.

Download the EmbryoCellect® General Information

Download the EmbryoCellect® Technical Information

Why EmbryoCellect™?

Samples can be processed in batches as small as four, which makes the workflow more flexible

The EmbryoCellect™ kit contents

Each EmbryoCellect® kit contains reagents for analysing 20 test samples:

  • Cell lysis buffer and enzyme for lysing the sample cells
  • Reference male gDNA
  • Whole Genome Amplification (WGA) reagents
  • Fluorescent labelling PCR reagents
  • Five patented EmbryoCellect® microarray slides with four microarrays per slide
  • Software: A Simple Excel macro that uses a microarray scanner-generated data file

The EmbryoCellect® workflow

Protocol Step Explanation
Cell lysis

Following biopsy, a gentle but effective enzyme-based lysis procedure ensures robust cell lysis and a readily accessible DNA template for whole genome amplification - 15 mins

Whole genome amplification

Whole genome amplification is performed using RHS’ DOP-PCR, which has been optimised for the RHS microarray. DOP-PCR uses degenerate primers to initiate DNA amplification, binding across a broad range of different sequences scattered genome wide -2 ½ hours

Agarose gel assessment

Following amplification, the use of agarose gel electrophoresis is recommended to ensure that cell amplification has been successful - 30 mins

Labelling PCR

Successfully amplified samples are fluorescently labelled by a second DOP-PCR. The test is labelled with a Cy3 equivalent dye and the reference with a Cy5 equivalent dye - 45 mins

Clean-up and nanodrop Agarose gel assessment

Once purified, these labelled amplicons are again assessed using agarose gel electrophoresis and spectrophotometry to ensure adequate amplification and dye incorporation has occurred - 1 hour

Hybridisation

Microarray washing, Microarray scanning and analysis

Samples are competitively hybridized to the RHS microarray - 3 hours, to overnight

After incubation, the microarray is washed and scanned (1 hour). The ratio of test to reference dye intensity after normalization is determined using RHS proprietary analysis method, providing the ploidy status of each chromosome in each sample.

For more information on the EmbryoCellect® kit, infrastructure, equipment and staff requirements

please contact us

Sign up for updates