RHS Poster Presentation at ESHRE 2016 Helsinki
Jul 05, 2016 -
RHS are currently in Helsinki at the ESHRE Conference for 2016 and have been selected to present their poster titled 'Next Generation Sequencing (NGS) metrics following DOP-PCR whole genome amplification (WGA) of single and multi-cell samples for PGS', prepared in conjunction with the Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, NSW, and the School of Women's & Children's Health, University of NSW.
The aim of the study was to compare Next Generation Sequencing (NGS) workflows and aligned read data metrics from a range of NGS platforms as models for Pre-implantation Genetic Screening (PGS) and Preimplantation Genetic Diagnosis (PGD). Comparisons were made using single cell and 5-cell aliquots amplified utilising the Reproductive Health Science Ltd proprietary DOP-PCR based WGA as described in the DOPlify™ and EmbryoCellect™ kits. Cell aneuploidy and mitochondrial DNA load was determined in addition to the evaluation of allele-drop out and the presence of genes of clinical interest.